Journal: Journal of Nanobiotechnology

Article Title: Young small extracellular vesicles rejuvenate replicative senescence by remodeling Drp1 translocation-mediated mitochondrial dynamics

doi: 10.1186/s12951-024-02818-5

Figure Lengend Snippet: Impaired Drp1-mediated mitochondrial dynamism induces a senescent-like phenomenon in BMSCs. a Colocalization analysis of Drp1 (green) and mitochondria (red). Scale bar, 10 μm. MOC were analyzed by Zeiss LSM 980. b RT-qPCR assay of Drp1. c Representative images of SA-β-gal positive BMSCs. Scale bar, 50 μm. d Quantification of SA-β-gal positive BMSCs. e Representative images of EdU-positive BMSCs. Scale bar, 50 μm. f Quantification of EdU-positive BMSCs. g Representative images of ALP staining of BMSCs treated as indicated after osteogenic induction for 7 days. Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns: not significant ( p > 0.05)

Article Snippet: After fixation and blocking, cells were incubated with rabbit anti-human Drp1 primary antibody (1:100, BOSTER) in a wet box overnight at 4 °C.

Techniques: Quantitative RT-PCR, Staining

Journal: Journal of Nanobiotechnology

Article Title: Young small extracellular vesicles rejuvenate replicative senescence by remodeling Drp1 translocation-mediated mitochondrial dynamics

doi: 10.1186/s12951-024-02818-5

Figure Lengend Snippet: Young sEVs rejuvenate replicative senescent BMSCs by promoting Drp1 translocation onto mitochondria. a SER texture analysis in BMSCs. Scale bar, 50 μm. b Analysis of mitochondrial membrane potential (MMP) using JC-1 staining. Quantification of depolarized cells was calculated. c Quantification of ATP levels per group. d Quantification of the intercellular ROS levels using mean fluorescence intensity of the DCFH-DA. e Quantification of the mitoSOX-positive mitochondria in all mitochondria. f Representative images of western blot analysis showing the expression levels of Drp1 and β-actin. g Representative images of colocalization of mitochondria (red), and Drp1 (green) per group. Scale bar, 10 μm. h Representative images of western blot analysis showing the change of mitochondrial fission gene Drp1. Data is shown as mean ± SEM. Comparison with P3 BMSCs: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns: no significant difference. Comparisons between identical passage: # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001. NS: no significant difference

Article Snippet: After fixation and blocking, cells were incubated with rabbit anti-human Drp1 primary antibody (1:100, BOSTER) in a wet box overnight at 4 °C.

Techniques: Translocation Assay, Membrane, Staining, Fluorescence, Western Blot, Expressing, Comparison